The video 'Roots of Resistance: How Highways Segregated American Cities' delves into the effects of the United States Interstate Highway System on urban communities, especially those predominantly inhabited by African-Americans. It explores historical urban displacement, chronicled by the destruction of neighborhoods, such as Rondo in Saint Paul, Minnesota. A critical theme is the examination of 'urban renewal' policies and their contributions to racial segregation through infrastructure development. The narrative covers key figures like Robert Moses, whose projects prioritized highway construction over community welfare, leading to widespread displacement. This content underscores the link between highway development and systemic racism, examining grassroots movements like the 'People Before Highways' campaign in Boston. Highlighting legislative reforms, such as the National Environmental Policy Act, demonstrates ongoing efforts to rectify past damages. The video is illuminating for understanding urban planning, civil rights, and the socio-economic impacts of infrastructure on marginalized communities.